Scientists have been conducting research over the past years to determine the role of cannabinoids when it comes to bone healing and development. The research found that the compound cannabidiol (CBD), which is non-psychoactive and is found in hemp plant, has different medical benefits like reducing seizure disorder, mood disorder, and stress and anxiety disorder. CBD oil for pain is also widely used you can find out more information from comments below.
Along with these many benefits, studies have been conducted to determine CBD’s involvement in bone fracture healing. One of the discoveries is that CBD helps heal bone fractures and stimulate the Lysyl Hydroxylase enzyme in the process of bone healing.
The study was conducted by researchers at the Tel Aviv University and Hebrew University of Jerusalem. The research was done by 18 scientists from Zurich, Sweden and Israeli. Dr. Raphael Mechoulam, the scientist who founded Tetrahydrocannabinol (THC) around 50 years ago, was also present. The research also included Yankel Gabet, a bone researcher at faculty of medicine in Tel Aviv University.
The study was performed with three different experiments. The first experiment involved injecting THC or CBD into rats with broken femurs or broken thighbones. This experiment was to determine the effects of THC or CBD on the mechanical and structural properties of fracture healing.
The second experiment involved injecting CBD and THC of equal amounts on a fractured callus which is a cartilage bridge that forms when bone is on the mend. The third experiment sought to find out THC and CBD’s effects on Osteoblast (bone building cells) and enzyme lysyl hydroxylase that promotes bone healing.
In the first and second experiments, the Fourier Transform Infrared Spectroscopy (FTIS) was used to monitor the bone’s molecular composition for eight weeks.
The fractures underwent mechanical testing, and a Micro-computed tomography was used. In order to determine the bone mineralization or density of mineralized callus, the pins stabilizing the fracture had to be removed. Then, toughness was tested by twisting the bone 5millimeters per minute till it fractured again.
In the third experiment, the osteoblasts of newborn mouse were cultured for four days at 37° Celsius. Once the culture had attained 80% maturation, THC and CBD were then added to it. Then, the RNA was extracted to examine the abundance of its lysyl hydroxylase was tested.
In experiment number 1, researchers found out that fracture callus increased in size in rats that were injected with CBD or THC. However, the callus size did not increase in rats with controlled substance. The results were studied after 6-8 weeks.
In general, the researchers found out that CBD enhanced bone healing by strengthening the fracture callus. It was also determined that the rate of bone healing was improved when the endocannabinoid receptors on bone cells was stimulated with cannabis ligands.
In experiment number 2, both THC and CBD were administered together in equal amounts. After eight weeks, it was revealed that THC increased the maximal force as well as the stiffness of the bones a little more than CBD did.
In experiment number 3, CBD was found to increase the mRNA level of the lysyl hydroxylase gene which is part of the fractured cross bridge formation. In fact, this is one of the first recorded data that offers insight to both scientists and consumers on the impact of marijuana on the body. It gives us a deeper understanding of how marijuana influences the body’s psychology since everything starts with genetics.
These studies have brought to light the wide variety of applications of CBD with regard to bone fracture healing. CBD can be used to treat bone-related conditions like Osteoporosis which weakens the bones and affects about 54 million people in the U.S. Another interesting use for CBD is stimulating the bone-building cells needed in the bone healing process. It also has an involvement in the increase of the mRNA levels of lysyl hydroxylase gene which is involved in fracture cross bridge formation.